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phosphorylated mapk14 p mapk14  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phosphorylated mapk14 p mapk14
    Phosphorylated Mapk14 P Mapk14, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+mapk14/pm41232727-74-0-6
    Average 86 stars, based on 1 article reviews
    phosphorylated mapk14 p mapk14 - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Rehmannioside A alleviates renal inflammation and fibrosis in hypertensive nephropathy via AT1R/MAPK14/IL-17 signaling pathway
    Article Snippet: Background: This study aimed to observe the influences and potential mechanism of rehmannioside A (ReA) in hypertensive nephropathy (HN).. Methods: HN model in mice and rat tubular epithelial cells were constructed by angiotensin II (Ang II).. The biomarkers of renal function, including uric acid (UA), creatinine (Cre), blood urea nitrogen (BUN), and urine albumin, were assessed.

    Membrane:

    Article Title: Rehmannioside A alleviates renal inflammation and fibrosis in hypertensive nephropathy via AT1R/MAPK14/IL-17 signaling pathway
    Article Snippet: Background: This study aimed to observe the influences and potential mechanism of rehmannioside A (ReA) in hypertensive nephropathy (HN).. Methods: HN model in mice and rat tubular epithelial cells were constructed by angiotensin II (Ang II).. The biomarkers of renal function, including uric acid (UA), creatinine (Cre), blood urea nitrogen (BUN), and urine albumin, were assessed.

    Incubation:

    Article Title: Rehmannioside A alleviates renal inflammation and fibrosis in hypertensive nephropathy via AT1R/MAPK14/IL-17 signaling pathway
    Article Snippet: Background: This study aimed to observe the influences and potential mechanism of rehmannioside A (ReA) in hypertensive nephropathy (HN).. Methods: HN model in mice and rat tubular epithelial cells were constructed by angiotensin II (Ang II).. The biomarkers of renal function, including uric acid (UA), creatinine (Cre), blood urea nitrogen (BUN), and urine albumin, were assessed.

    Article Title: Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation
    Article Snippet: The samples derived from cells and tissue were lysed, separated by electrophoresis on SDS-PAGE gels (Beyotime Institute of Biotechnology, P0012AC) and transferred to polyvinylidene fluoride (PVDF) membranes (MerckMinipore, IPVH00010 or ISEQ00010). .. For western blotting detection, the proteins were incubated overnight with the following primary antibodies: BECN1 (1:1000 dilution), LC3A/B-II (1:1000 dilution), LC3A-II (1:1000 dilution), LC3B-II (1:1000 dilution), ATG5 (Sigma‐Aldrich, A0731; 1:1000 dilution), SQSTM1 (Cell Signaling Technology, 5114S; 1:1000 dilution), HMGB1 (1:1000 dilution), p-MAPK8/9/10 (Cell Signaling Technology, 9251S; 1:1000 dilution), MAPK8/9/10 (Cell Signaling Technology, 9252S; 1:1000 dilution), p-MAPK1/3 (Cell Signaling Technology, 9101S; 1:1000 dilution), MAPK1/3 (Cell Signaling Technology, 9102S; 1:1000 dilution), p-MAPK14 (Cell Signaling Technology, 9211S; 1:1000 dilution), MAPK14 (Cell Signaling Technology, 9212S; 1:1000 dilution), RELA/p65 (Cell Signaling Technology, 8242S; 1:1000 dilution), Histone H3 (Cell Signaling Technology, 4499S; 1:2000 dilution), PRO-CASP1 (R&D Systems, MAB6215; 0.1 μg/mL), CASP1 p20 (Adipogen, AG-20B-0048-C100; 1:1000 dilution), and ACTB (actin beta; Proteintech group, 60008-1-Ig; 1:10000 dilution), incubated overnight. .. Labeling of the primary antibodies was detected using goat anti-rabbit or goat anti-mouse antibodies conjugated to horseradish peroxidase (HRP) (ZSGB-BIO, ZB-2301 or ZB2305; 1:10000 dilution), and further detected using ECL reagents (MerckMinipore, WBULS0500).

    Article Title: Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation.
    Article Snippet: The samples derived from cells and tissue were lysed, separated by electrophoresis on SDS-PAGE gels (Beyotime Institute of Biotechnology, P0012AC) and transferred to polyvinylidene fluoride (PVDF) membranes (MerckMinipore, IPVH00010 or ISEQ00010). .. For western blotting detection, the proteins were incubated overnight with the following primary antibodies: BECN1 (1:1000 dilution), LC3A/B-II (1:1000 dilution), LC3A-II (1:1000 dilution), LC3B-II (1:1000 dilution), ATG5 (Sigma‐Aldrich, A0731; 1:1000 dilution), SQSTM1 (Cell Signaling Technology, 5114S; 1:1000 dilution), HMGB1 (1:1000 dilution), p-MAPK8/9/10 (Cell Signaling Technology, 9251S; 1:1000 dilution), MAPK8/9/10 (Cell Signaling Technology, 9252S; 1:1000 dilution), p-MAPK1/3 (Cell Signaling Technology, 9101S; 1:1000 dilution), MAPK1/3 (Cell Signaling Technology, 9102S; 1:1000 dilution), p-MAPK14 (Cell Signaling Technology, 9211S; 1:1000 dilution), MAPK14 (Cell Signaling Technology, 9212S; 1:1000 dilution), Ac ce pte d M an us cri pt RELA/p65 (Cell Signaling Technology, 8242S; 1:1000 dilution), Histone H3 (Cell Signaling Technology, 4499S; 1:2000 dilution), PRO-CASP1 (R&D Systems, MAB6215; 0.1 μg/mL), CASP1 p20 (Adipogen, AG-20B-0048-C100; 1:1000 dilution), and ACTB (actin beta; Proteintech group, 60008-1-Ig; 1:10000 dilution), incubated overnight. .. Labeling of the primary antibodies was detected using goat anti-rabbit or goat anti-mouse antibodies conjugated to horseradish peroxidase (HRP) (ZSGB-BIO, ZB-2301 or ZB2305; 1:10000 dilution), and further detected using ECL reagents (MerckMinipore, WBULS0500).

    Ubiquitin Proteomics:

    Article Title: Rehmannioside A alleviates renal inflammation and fibrosis in hypertensive nephropathy via AT1R/MAPK14/IL-17 signaling pathway
    Article Snippet: Background: This study aimed to observe the influences and potential mechanism of rehmannioside A (ReA) in hypertensive nephropathy (HN).. Methods: HN model in mice and rat tubular epithelial cells were constructed by angiotensin II (Ang II).. The biomarkers of renal function, including uric acid (UA), creatinine (Cre), blood urea nitrogen (BUN), and urine albumin, were assessed.

    Western Blot:

    Article Title: Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation
    Article Snippet: The samples derived from cells and tissue were lysed, separated by electrophoresis on SDS-PAGE gels (Beyotime Institute of Biotechnology, P0012AC) and transferred to polyvinylidene fluoride (PVDF) membranes (MerckMinipore, IPVH00010 or ISEQ00010). .. For western blotting detection, the proteins were incubated overnight with the following primary antibodies: BECN1 (1:1000 dilution), LC3A/B-II (1:1000 dilution), LC3A-II (1:1000 dilution), LC3B-II (1:1000 dilution), ATG5 (Sigma‐Aldrich, A0731; 1:1000 dilution), SQSTM1 (Cell Signaling Technology, 5114S; 1:1000 dilution), HMGB1 (1:1000 dilution), p-MAPK8/9/10 (Cell Signaling Technology, 9251S; 1:1000 dilution), MAPK8/9/10 (Cell Signaling Technology, 9252S; 1:1000 dilution), p-MAPK1/3 (Cell Signaling Technology, 9101S; 1:1000 dilution), MAPK1/3 (Cell Signaling Technology, 9102S; 1:1000 dilution), p-MAPK14 (Cell Signaling Technology, 9211S; 1:1000 dilution), MAPK14 (Cell Signaling Technology, 9212S; 1:1000 dilution), RELA/p65 (Cell Signaling Technology, 8242S; 1:1000 dilution), Histone H3 (Cell Signaling Technology, 4499S; 1:2000 dilution), PRO-CASP1 (R&D Systems, MAB6215; 0.1 μg/mL), CASP1 p20 (Adipogen, AG-20B-0048-C100; 1:1000 dilution), and ACTB (actin beta; Proteintech group, 60008-1-Ig; 1:10000 dilution), incubated overnight. .. Labeling of the primary antibodies was detected using goat anti-rabbit or goat anti-mouse antibodies conjugated to horseradish peroxidase (HRP) (ZSGB-BIO, ZB-2301 or ZB2305; 1:10000 dilution), and further detected using ECL reagents (MerckMinipore, WBULS0500).

    Article Title: Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation.
    Article Snippet: The samples derived from cells and tissue were lysed, separated by electrophoresis on SDS-PAGE gels (Beyotime Institute of Biotechnology, P0012AC) and transferred to polyvinylidene fluoride (PVDF) membranes (MerckMinipore, IPVH00010 or ISEQ00010). .. For western blotting detection, the proteins were incubated overnight with the following primary antibodies: BECN1 (1:1000 dilution), LC3A/B-II (1:1000 dilution), LC3A-II (1:1000 dilution), LC3B-II (1:1000 dilution), ATG5 (Sigma‐Aldrich, A0731; 1:1000 dilution), SQSTM1 (Cell Signaling Technology, 5114S; 1:1000 dilution), HMGB1 (1:1000 dilution), p-MAPK8/9/10 (Cell Signaling Technology, 9251S; 1:1000 dilution), MAPK8/9/10 (Cell Signaling Technology, 9252S; 1:1000 dilution), p-MAPK1/3 (Cell Signaling Technology, 9101S; 1:1000 dilution), MAPK1/3 (Cell Signaling Technology, 9102S; 1:1000 dilution), p-MAPK14 (Cell Signaling Technology, 9211S; 1:1000 dilution), MAPK14 (Cell Signaling Technology, 9212S; 1:1000 dilution), Ac ce pte d M an us cri pt RELA/p65 (Cell Signaling Technology, 8242S; 1:1000 dilution), Histone H3 (Cell Signaling Technology, 4499S; 1:2000 dilution), PRO-CASP1 (R&D Systems, MAB6215; 0.1 μg/mL), CASP1 p20 (Adipogen, AG-20B-0048-C100; 1:1000 dilution), and ACTB (actin beta; Proteintech group, 60008-1-Ig; 1:10000 dilution), incubated overnight. .. Labeling of the primary antibodies was detected using goat anti-rabbit or goat anti-mouse antibodies conjugated to horseradish peroxidase (HRP) (ZSGB-BIO, ZB-2301 or ZB2305; 1:10000 dilution), and further detected using ECL reagents (MerckMinipore, WBULS0500).

    other:

    Article Title: Combination of autophagy and NFE2L2/NRF2 activation as a treatment approach for neuropathic pain
    Article Snippet: p-MAPK14 , Cell Signaling Technology, USA , 9215 , IB: 1:1000.

    Article Title: Elastase induces lung epithelial cell autophagy through placental growth factor
    Article Snippet: Reagents Rabbit antibodies for SQSTM1 (8025), MAP1LC3B (3868), phospho-MAPK8 (p-MAPK8) (4668), p-MAPK14 (9215), MAPK14 (9212), p-TSC2 (3617) and p-MTOR (2971) and human ATG5 siRNA (6345) were obtained from Cell Signaling Technology.



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    Effect of BBR intervention on protein expression in DKD rats by Western blot. ( A and B ) Western blot analysis and quantification of proteins in the IL-17A/TRAF6/MAPK14 pathway in kidney tissues. *P < 0.05, # P > 0.05 vs the model group.

    Journal: Journal of Inflammation Research

    Article Title: Berberine Ameliorates Diabetic Kidney Disease by Modulating Macrophage Polarization via Inhibiting IL-17A Signaling

    doi: 10.2147/JIR.S580534

    Figure Lengend Snippet: Effect of BBR intervention on protein expression in DKD rats by Western blot. ( A and B ) Western blot analysis and quantification of proteins in the IL-17A/TRAF6/MAPK14 pathway in kidney tissues. *P < 0.05, # P > 0.05 vs the model group.

    Article Snippet: These membranes were subsequently incubated overnight at 4°C with primary antibodies specifically targeting IL17A (Proteintech, 26163-1-AP, 1:3000), MCP-1 (Proteintech, 26161-1-AP, 1:2000), MMP9 (Proteintech, 10375-2-AP, 1:3000), TRAF6 (ABclonal, A23385, 1:10,000), MAPK14 (Proteintech, 14064-1-AP, 1:10,000), p-MAPK14 (Proteintech, 28796-1-AP, 1:3000), Caspase-3 (Proteintech, 19677-1-AP, 1:3000), Bcl-2 (Proteintech, 26593-1-AP, 1:3000), and Bax (Proteintech, 50599-2-lg, 1:10,000).

    Techniques: Expressing, Western Blot